Figure 4.

Perinatal fentanyl exposure impairs synaptic transmission in anterior cingulate cortical neurons. A, Timeline depicting slice electrophysiology recordings in ACC layer 5 neurons of adolescent mice. B, Example traces of mEPSCs. C, Cumulative frequency plot of the interevent intervals of mEPSCs are shifted to the left in fentanyl-exposed mice compared with controls. D, Grouped data reflect increased mEPSC frequency in fentanyl-exposed mice. E, Cumulative frequency of the mEPSC amplitude was shifted to the right in fentanyl-exposed mice. F, There were no differences in grouped data of mEPSC amplitudes. G, Example traces of evoked paired pulse and NMDAR-mediated responses. H, Perinatal fentanyl exposure results in decreased paired pulse ratio in fentanyl-exposed mice. There were no differences in AMPAR-mediated response amplitudes (I), NMDAR-mediated response amplitudes (J), or the NMDA/AMPA ratio (K). L, Example traces of mIPSCs. M, Cumulative frequency plot of the interevent intervals of mIPSCs are shifted to the left in fentanyl-exposed mice compared with controls. N, There were no differences in grouped data of mIPSC frequency. O, Cumulative frequency plot of the mIPSC amplitude was shifted to the right. P, There were no differences in grouped data of mIPSC amplitude. Data depict means for parametric or medians for non-parametric comparisons with 95% confidence intervals.