Table 1.

mRNA expression of receptors involved in synaptic transmission

mRNA	Region	Vehicle				Fentanyl				Analysis	p value	p < 0.05
		n	Mean	Median	95% CI	n	Mean	Median	95% CI
CB1R	S1	14	1.00	0.99	0.92–1.07	13	1.15	1.14	1.03–1.27	t test	0.02	Yes
	ACC	13	1.00	0.96	0.91–1.08	14	0.86	0.85	0.78–0.93	t test	0.01	Yes
GABAB1	S1	14	1.00	1.01	0.91–1.08	13	1.01	1.05	0.89–1.14	t test	0.78	No
	ACC	13	1.00	0.98	0.95–1.04	13	0.90	0.94	0.84–0.97	t test	0.01	Yes
GABAB2	S1	13	1.00	0.97	0.89–1.10	12	1.04	1.07	0.82–1.27	Mann–Whitney	0.46	No
	ACC	13	1.00	0.95	0.91–1.08	14	1.02	1.01	0.85–1.19	t test	0.77	No
mGluR1	S1	13	1.00	1.08	0.82–1.17	13	0.93	0.93	0.66–1.19	t test	0.64	No
	ACC	13	1.00	0.99	0.94–1.05	14	0.73	0.70	0.65–0.81	t test	<10−4	Yes
mGluR2	S1	14	1.00	1.06	0.88–1.12	13	1.01	0.94	0.87–1.15	t test	0.89	No
	ACC	13	1.00	1.05	0.91–1.08	14	0.84	0.83	0.75–0.94	t test	0.01	Yes
mGluR3	S1	14	1.00	0.99	0.93–1.06	13	1.04	1.02	0.93–1.16	t test	0.44	No
	ACC	13	1.00	0.98	0.91–1.08	14	0.89	0.88	0.83–0.95	t test	0.04	Yes
mGluR4	S1	14	1.00	0.89	0.83–1.16	13	0.94	0.85	0.80–1.09	t test	0.59	No
	ACC	13	1.00	1.02	0.90–1.09	14	0.90	0.91	0.80–1.00	t test	0.15	No
mGluR5	S1	14	1.00	0.98	0.88–1.11	13	1.01	0.95	0.86–1.16	t test	0.88	No
	ACC	13	1.00	1.04	0.91–1.08	14	0.88	0.79	0.70–1.06	t test	0.22	No
mGluR7	S1	14	1.00	1.06	0.79–1.20	13	1.04	1.13	0.79–1.29	t test	0.75	No
	ACC	13	1.00	0.99	0.82–1.17	14	1.21	0.79	0.68–1.74	t test	0.41	No
mGluR8	S1	14	1.00	0.90	0.81–1.18	13	0.74	0.74	0.69–0.80	Mann–Whitney	0.01	Yes
	ACC	13	1.00	0.91	0.85–1.14	14	0.83	0.86	0.73–0.94	t test	0.05	No
GluR1	S1	14	1.00	0.99	0.90–1.09	13	0.82	0.78	0.65–0.98	t test	0.04	Yes
	ACC	13	1.00	0.96	0.83–1.16	14	0.90	0.90	0.78–1.02	t test	0.30	No
GluR2	S1	14	1.00	0.98	0.94–1.05	13	0.92	0.86	0.76–1.08	Mann–Whitney	0.16	No
	ACC	13	1.00	1.04	0.85–1.14	14	0.99	0.93	0.85–1.13	t test	0.95	No
GluR3	S1	14	1.00	0.99	0.91–1.08	13	0.84	0.78	0.66–1.02	Mann–Whitney	0.03	Yes
	ACC	13	1.00	1.01	0.86–1.13	14	0.83	0.91	0.72–0.95	t test	0.06	No
GluR4	S1	14	1.00	0.95	0.92–1.07	13	0.89	0.88	0.79–1.00	t test	0.08	No
	ACC	13	1.00	0.96	0.91–1.09	14	1.00	1.05	0.87–1.14	t test	0.92	No
GluN2A	S1	14	1.00	1.03	0.89–1.10	13	0.94	0.99	0.84–1.05	t test	0.46	No
	ACC	13	1.00	1.01	0.91–1.08	14	0.87	0.91	0.81–0.93	t test	0.01	Yes
GluN2B	S1	14	1.00	1.00	0.88–1.11	13	1.03	0.94	0.78–1.28	Mann–Whitney	0.75	No
	ACC	12	1.00	0.92	0.86–1.07	12	0.86	0.83	0.77–0.94	t test	0.09	No
GluN2C	S1	14	1.00	1.00	0.91–1.08	12	1.28	1.34	1.09–1.48	Mann–Whitney	0.01	Yes
	ACC	13	1.00	1.08	0.86–1.13	14	0.90	0.81	0.72–1.09	t test	0.41	No
GluN2D	S1	14	1.00	0.99	0.85–1.14	13	0.93	0.93	0.80–1.06	t test	0.50	No
	ACC	13	1.00	1.05	0.90–1.09	13	0.64	0.61	0.56–0.72	t test	<10−4	Yes