Table 1.
Gene | Primer sequence |
---|---|
miR‐24‐3p | F: 5′‐TGGCTCAGTTCAGCAGGAACAG‐3′ |
F: 5′‐ACCGAGTCAAGTCGTCCTTGTC‐3′ | |
U6 | F: 5′‐CGAGCACAGAATCGCTTCA‐3′ |
F: 5′‐CTCGCTTCGGCAGCACATAT‐3′ | |
HEPH | F: 5′‐TCCAATCGAATGCATGCATGCT‐3′ |
R: 5′‐AACATAACCCATGTACA‐3′ | |
CDX2 | F: 5′‐GATGATACTGCTGGGTACTG‐3′ |
R: 5′‐TCTCCTTTGCTCTGCGGTTC‐3′ | |
GAPDH | F: 5′‐GCACCGTCAAGGCTGAGAAC‐3′ |
R: 5′‐TGGTGAAGACGCCAGTGGA‐3′ |
Abbreviations: CDX2, caudal‐related homeobox 2; F, forward; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; HEPH, hephaestin; miR‐24‐3p, microRNA‐24‐3p; R, reverse; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction.