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. 2021 Feb 23;25(8):3699–3713. doi: 10.1111/jcmm.15765

Table 1.

Primer sequences for RT‐qPCR

Gene Primer sequence
miR‐24‐3p F: 5′‐TGGCTCAGTTCAGCAGGAACAG‐3′
F: 5′‐ACCGAGTCAAGTCGTCCTTGTC‐3′
U6 F: 5′‐CGAGCACAGAATCGCTTCA‐3′
F: 5′‐CTCGCTTCGGCAGCACATAT‐3′
HEPH F: 5′‐TCCAATCGAATGCATGCATGCT‐3′
R: 5′‐AACATAACCCATGTACA‐3′
CDX2 F: 5′‐GATGATACTGCTGGGTACTG‐3′
R: 5′‐TCTCCTTTGCTCTGCGGTTC‐3′
GAPDH F: 5′‐GCACCGTCAAGGCTGAGAAC‐3′
R: 5′‐TGGTGAAGACGCCAGTGGA‐3′

Abbreviations: CDX2, caudal‐related homeobox 2; F, forward; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; HEPH, hephaestin; miR‐24‐3p, microRNA‐24‐3p; R, reverse; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction.