Table 2. Sensitivity, accuracy and precision of UPT-LF, ELISA and HPLC-MS/MS methods for detection of multiple mycotoxins.
| Mycotoxina | Method | LODb (μg/kg) | Mean recovery (%) | Minimum recovery (%) | Maximum recovery (%) | RSDc (%) |
|---|---|---|---|---|---|---|
| AFB1 | UPT-LF | 3 | 104.39±14.21 | 81.71 | 127.23 | 13.96 |
| ELISA | 2 | 101.75±8.99 | 87.94 | 124.64 | 8.89 | |
| HPLC-MS/MS | 0.001 | 100.88±2.67 | 92.50 | 104.66 | 2.67 | |
| ZEN | UPT-LF | 50 | 102.94±15.40 | 76.47 | 129.24 | 13.71 |
| ELISA | 25 | 99.23±8.69 | 83.51 | 116.93 | 8.93 | |
| HPLC-MS/MS | 0.01 | 101.46±2.72 | 96.59 | 105.02 | 1.89 | |
| DON | UPT-LF | 200 | 103.65±12.65 | 79.06 | 122.50 | 12.56 |
| ELISA | 250 | 100.43±7.82 | 89.00 | 115.55 | 8.02 | |
| HPLC-MS/MS | 1 | 100.22±2.34 | 94.85 | 104.97 | 2.80 |
Means with different superscripts are significantly different within a column (P < 0.05). Data are expressed as Mean ± SD (n = 15).
aAFB1, aflatoxin B1; ZEN, zearalenone; DON, deoxynivalenol.
bLOD, limit of detection.
cRSD, relative standard deviation.