Table 7. Aflatoxin B1 contamination in feed mixtures measured by UPT-LF, ELISA and HPLC-MS/MS methods.
| Feed mixture | Method | Positive rate (%) | Mean value (μg/kg) | Median value (μg/kg) | Maximum value (μg/kg) |
|---|---|---|---|---|---|
| Pig concentrated feed | UPT-LF | 100 | 7.33±0.19 | 7.33 | 7.54 |
| ELISA | 100 | 9.43±2.87 | 11.05 | 11.95 | |
| HPLC-MS/MS | 100 | 9.16±2.80 | 10.62 | 11.48 | |
| Laying hen concentrated feed | UPT-LF | 100 | 35.84±13.50 | 29.66 | 54.17 |
| ELISA | 100 | 24.27±9.43 | 22.73 | 35.73 | |
| HPLC-MS/MS | 100 | 23.00±10.78 | 19.30 | 37.12 | |
| Broiler complete feed | UPT-LF | 100 | 19.65±6.38 | 22.01 | 24.87 |
| ELISA | 100 | 22.36±9.48 | 20.34 | 34.33 | |
| HPLC-MS/MS | 100 | 17.68±8.02 | 15.52 | 30.61 | |
| Cow complete feed | UPT-LF | 100 | 36.51±10.16 | 33.69 | 51.88 |
| ELISA | 100 | 26.07±7.67 | 24.88 | 38.95 | |
| HPLC-MS/MS | 100 | 26.61±8.00 | 23.03 | 39.51 | |
| Pig complete feed | UPT-LF | 100 | 7.26±1.77 | 6.95 | 10.27 |
| ELISA | 100 | 6.64±1.69 | 6.38 | 9.53 | |
| HPLC-MS/MS | 100 | 5.86±1.52 | 5.71 | 8.22 | |
| Meat duck complete feed | UPT-LF | 60 | 10.59±12.26 | 22.43 | 25.09 |
| ELISA | 100 | 15.07±3.39 | 11.02 | 27.34 | |
| HPLC-MS/MS | 100 | 14.92±7.88 | 11.19 | 28.56 | |
| Laying hen complete feed | UPT-LF | 100 | 30.22±3.72a | 29.57 | 34.94 |
| ELISA | 100 | 17.79±4.18b | 17.59 | 21.73 | |
| HPLC-MS/MS | 100 | 17.85±4.02b | 17.75 | 21.64 |
Means with different superscripts are significantly different within a column (P < 0.05). Data are expressed as Mean ± SD (n = 5).