Table 8. Zearalenone contamination in feed mixtures measured by UPT-LF, ELISA and HPLC-MS/MS methods.
| Feed mixture | Method | Positive rate (%) | Mean value (μg/kg) | Median value (μg/kg) | Maximum value (μg/kg) |
|---|---|---|---|---|---|
| Pig concentrated feed | UPT-LF | 100 | 73.63±9.98 | 68.83 | 88.97 |
| ELISA | 100 | 73.35±34.10 | 87.92 | 109.40 | |
| HPLC-MS/MS | 100 | 84.96±21.97 | 79.49 | 110.98 | |
| Laying hen concentrated feed | UPT-LF | 100 | 325.65±61.38 | 333.27 | 418.52 |
| ELISA | 100 | 224.05±109.74 | 246.84 | 338.82 | |
| HPLC-MS/MS | 100 | 238.05±122.33 | 253.80 | 394.23 | |
| Broiler complete feed | UPT-LF | 100 | 76.75±29.83 | 63.45 | 128.85 |
| ELISA | 100 | 92.96±27.15 | 91.60 | 135.33 | |
| HPLC-MS/MS | 100 | 78.51±31.99 | 99.63 | 103.94 | |
| Cow complete feed | UPT-LF | 100 | 81.89±20.97 | 73.79 | 119.02 |
| ELISA | 100 | 88.05±34.49 | 98.62 | 126.23 | |
| HPLC-MS/MS | 100 | 103.97±27.66 | 88.82 | 150.17 | |
| Pig complete feed | UPT-LF | 60 | 41.56±42.18ab | 56.06 | 99.31 |
| ELISA | 100 | 71.12±16.77a | 67.62 | 99.16 | |
| HPLC-MS/MS | 100 | 29.04±3.35b | 27.94 | 34.83 | |
| Meat duck complete feed | UPT-LF | 100 | 103.14±19.09a | 93.94 | 127.65 |
| ELISA | 100 | 34.50±21.84b | 23.50 | 69.57 | |
| HPLC-MS/MS | 100 | 52.91±10.70b | 57.40 | 61.03 | |
| Laying hen complete feed | UPT-LF | 100 | 70.95±18.90a | 66.53 | 96.65 |
| ELISA | 100 | 34.92±9.17b | 29.85 | 49.07 | |
| HPLC-MS/MS | 100 | 43.33±3.25b | 42.96 | 48.19 |
Means with different superscripts are significantly different within a column (P < 0.05). Data are expressed as Mean ± SD (n = 5).