Table 9. Deoxynivalenol contamination in feed mixtures measured by UPT-LF, ELISA and HPLC-MS/MS methods.
| Feed mixture | Method | Positive rate (%) | Mean value (μg/kg) | Median value (μg/kg) | Maximum value (μg/kg) |
|---|---|---|---|---|---|
| Pig concentrated feed | UPT-LF | 80 | 482.50±246.52 | 520.00 | 680.00 |
| ELISA | 100 | 384.14±241.40 | 374.20 | 680.35 | |
| HPLC-MS/MS | 100 | 517.90±254.20 | 581.23 | 681.23 | |
| Laying hen concentrated feed | UPT-LF | 100 | 424.00±174.73 | 450.00 | 680.00 |
| ELISA | 100 | 398.01±130.45 | 453.28 | 512.69 | |
| HPLC-MS/MS | 100 | 445.24±192.66 | 462.53 | 642.30 | |
| Broiler complete feed | UPT-LF | 100 | 302.00±132.93 | 210.00 | 480.00 |
| ELISA | 100 | 221.95±134.60 | 150.12 | 421.37 | |
| HPLC-MS/MS | 100 | 345.27±125.51 | 271.03 | 521.94 | |
| Cow complete feed | UPT-LF | 100 | 858.00±427.22 | 970.00 | 1230.00 |
| ELISA | 100 | 825.11±461.80 | 990.34 | 1231.72 | |
| HPLC-MS/MS | 100 | 806.09±446.44 | 980.34 | 1233.67 | |
| Pig complete feed | UPT-LF | 100 | 276.00±67.31a | 260.00 | 380.00 |
| ELISA | 100 | 209.14±41.89b | 180.34 | 256.31 | |
| HPLC-MS/MS | 100 | 304.86±50.30a | 331.28 | 356.91 | |
| Meat duck complete feed | UPT-LF | 100 | 204.00±145.02 | 200.00 | 410.00 |
| ELISA | 100 | 210.82±136.39 | 174.20 | 450.36 | |
| HPLC-MS/MS | 100 | 202.24±115.80 | 145.31 | 403.69 | |
| Laying hen complete feed | UPT-LF | — | — | — | — |
| ELISA | — | — | — | — | |
| HPLC-MS/MS | 100 | 17.81±4.42 | 16.34 | 24.91 |
Means with different superscripts are significantly different within a column (P < 0.05). Data are expressed as Mean ± SD (n = 5).