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. 2021 Apr 1;15(4):e0009308. doi: 10.1371/journal.pntd.0009308

Fig 6. Passive Transfer of AdV-MAYV Immune Sera Protects Against Pathological Effects of Infection.

Fig 6

Naïve or immune sera was passively transferred to groups of five-week-old female WT C57BL/6N mice one day prior to challenge with 104 PFU MAYVBeAr in the right hind limb footpad. A second group of naïve five-week-old female WT C57BL/6N were mock inoculated with PBS. (A) Footpad swelling was monitored by digital calipers throughout the experiment. Statistical analysis was performed by paired repeated measures ANOVA (* P < 0.05, **** P < 0.0001). (B) Whole hind legs were harvested at 7 dpi and sectioned for histopathology by hematoxylin and eosin staining. Tissue sections were scored on a 0–5 scale using the no infection control tissues as a baseline score: 0 absent (no lesions), 1 minimal (1~10% of tissues affected), 2 mild (11~25% affected), 3 moderate (26~50% affected), 4 marked (51~75% affected), 5 severe (>75% affected). Statistical analysis was performed by two-way ANOVA (* P <0.05, **** P < 0.0001). Error bars represent SD.