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. 2021 Apr 16;7(16):eabf0753. doi: 10.1126/sciadv.abf0753

Fig. 4. SRSF1 regulates T cell development in a cell-intrinsic manner.

Fig. 4

(A) Flowchart of generation and analysis of BM chimeric mice. (B) Representative pseudocolor plot show the classic subsets of thymocytes with CD4 and CD8 staining from the chimeras generated with control and Srsf1fl/flLckCre/+ donor (CD45.2+) BM cells. (C) Frequency of indicated subsets as in (B) is shown accordingly (n = 6 per group). (D) Characterization of postselection thymocytes in chimeric mice. TCRβhi postselection thymocytes (CD45.2+) derived from control and Srsf1fl/flLckCre/+ mice in chimeras are shown by contour plot, and the gating strategies were consistent with those described in Fig. 1E. (E) Frequency of each subpopulation in (D) is statistically shown (n = 6 per group). Cumulative data are means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t test). Data are from at least three independent experiments.