Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
MEC13-53D | Blankvoort et al., 2018 | Not commercially available, but upon request we can send the mouse line. Send email to Dr. Stefan Blankvoort; stefan.blankvoort@ntnu.no. | |
| Strain, strain background (Mus musculus) | tetOGCaMP6-mCherry | Blankvoort et al., 2018 | Not commercially available, but upon request we can send the mouse line. Contact see above. | |
| Strain, strain background (Mus musculus) | GAD1GFP | Tamamaki et al., 2003 | The animals are bred in house after obtaining breeding pairs from Dr Yuchio Yanagawa;yanagawa@med.gunma-u.ac.jp. | |
| Strain, strain background (Mus musculus) | Gt(ROSA)26Sortm9(CAG-tdTomato)Hze | The Jackson Laboratory | 007909 RRID:IMSR_JAX:007909 |
|
| Genetic reagent (virus) | AAV-TRE-tight-GFP (serotype 2/1) | Nilssen et al., 2018 | Viral Vector Core at Kavli Institute for Systems Neuroscience; contact Dr Rajeevkumar Nair Raveendranrajeevkumar.r.nair@ntnu.no. | |
| Genetic reagent (virus) | AAV-TRE-tight-oChIEF-citrine (serotype 2/1) | Nilssen et al., 2018 | Viral Vector Core at Kavli Institute for Systems Neuroscience; contact see above. | |
| Genetic reagent (virus) | AAV-CMV-FLEX-mCherry (serotype 2/1) | This paper | Viral Vector Core at Kavli Institute for Systems Neuroscience; contact see above. | |
| Genetic reagent (virus) | AAVrg-pmSyn1-EBFP-cre | Addgene | 51507 | |
| Genetic reagent (virus) | AAV1.CAG.tdTomato.WPRE.SV40 | Upenn viral core | AV-1-PV3365 | |
| Antibody | Anti-GFP (chicken polyclonal) |
Abcam | ab13970 RRID:AB_300798 |
(1:500) |
| Antibody | Anti-GFP (rabbit polyclonal) |
Thermo Fisher Scientific | A11122 RRID:AB_221569 |
(1:2000) |
| Antibody | Anti-PCP4 (rabbit polyclonal) |
Sigma Aldrich | HPA005792 RRID:AB_1855086 |
(1:300) |
| Antibody | Anti-Ctip2 (rat monoclonal) |
Abcam | ab18465 RRID:AB_2064130 |
(1:3000) |
| Antibody | Anti-NeuN (guinea pig polyclonal) |
Millipore | ABN90P RRID:AB_2341095 |
(1:1000) |
| Antibody | Anti-NeuN (mouse monoclonal) |
Millipore | MAB377 RRID:AB_2298772 |
(1:1000) |
| Antibody | Anti-PHA-L (rabbit) |
Vector Laboratories | AS-2300 RRID:AB_2313686 |
(1:1000) |
| Antibody | Goat anti-chicken IgG (AF 488) | Thermo Fisher Scientific | A11039 RRID:AB_2534096 |
(1:400) |
| Antibody | Goat anti-rabbit IgG (AF 546) | Thermo Fisher Scientific | A11010 RRID:AB_2534077 |
(1:400) |
| Antibody | Goat anti-rabbit IgG (AF 635) | Thermo Fisher Scientific |
A31576 RRID:AB_10374303 |
(1:400) |
| Antibody | Goat anti-rat IgG (AF 633) | Thermo Fisher Scientific | A21094 RRID:AB_2535749 |
(1:400) |
| Antibody | Goat anti-guinea pig IgG (AF 647) | Thermo Fisher Scientific | A21450 RRID:AB_2735091 |
(1:400) |
| Antibody | Goat anti-guinea pig IgG (AF 488) | Thermo Fisher Scientific | A11073 RRID:AB_2534117 |
(1:400) |
| Antibody | Goat anti-mouse IgG (AF 488) | Thermo Fisher Scientific | A11001 RRID:AB_2534069 |
(1:400) |
| Antibody | Streptavidin, Alexa Fluor 546 conjugate | Thermo Fisher Scientific |
S11225 RRID:AB_2532130 |
(1:600) |
| Antibody | Cy3 streptavidin | Jackson Immuno Research | 016-160-084 RRID:AB_2337244 |
(1:400) |
| Antibody | Neurotrace 640/660 deep-red fluorescent Nissl stain | Thermo Fisher Scientific |
N21483 RRID:AB_2572212 |
(1:200) |
| Chemical compound, drug | Biotinylated dextran amine | Invitrogen | D1956 | |
| Chemical compound, drug | Phaseolus vulgaris leucoagglutinin | Vector Laboratories | L-1110 | |
| Software, algorithm | Patchmaster | Heka Eletronik | ||
| Software, algorithm | Clampfit | Molecular Devices | ||
| Software, algorithm | MATLAB, 2018a | MathWorks | ||
| Software, algorithm | Image J | http://rsb.info.nih.gov/ij | ||
| Software, algorithm | GraphPad Prism, version 5 | GraphPad software |