Appendix 1—table 1. Summary table of neurons in genetic drivers used, corresponding reconstructions in hemibrain connectome, and PSI data.
| Genetic driver | Hemibrain connectome v1.2 (Scheffer et al., 2020) | Average PSI† | Average ΔPSI(compared to control experiments with polarizing filter removed) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Designation | Neurons recorded (recording site) |
Estimated population size (per recording) | Corresponding neurons | Instances traced (per brain hemisphere) | Background | Neurons | p-value (t-test, background vs neurons) |
Background | p-value (t-test) |
Neurons | p-value (t-test) |
| DmDRA1-split | DmDRA1 (MEDRA) | 23 | n/a | n/a | 0.197 CI95 [0.18 0.21] N = 10 |
0.741 CI95 [0.70 0.78] N = 10 |
0.000000 | −0.002 CI95 [−0.02 0.01] N = 10 |
0.814673 | 0.586 CI95 [0.55 0.63] N = 10 |
0.000000 |
| R56F07 |
MeTuil + MeTul
(AOTU) |
~50–100 | MC61* | 347 | 0.209 CI95 [0.20 0.22] N = 17 |
0.483 CI95 [0.41 0.55] N = 17 |
0.000001 | 0.001 CI95 [−0.01 0.01] N = 17 |
0.836089 | 0.280 CI95 [0.21 0.35] N = 17 |
0.000000 |
| R73C04 |
MeTul + MeTuil
(AOTU) |
~100–150 | MC61* | 347 | 0.213 CI95 [0.18 0.25] N = 11 |
0.422 CI95 [0.29 0.56] N = 11 |
0.006841 | 0.000 CI95 [−0.03 0.03] N = 11 |
0.982761 | 0.242 CI95 [0.11 0.38] N = 11 |
0.002816 |
| R17F12 |
TuTu
(AOTU) |
2 | TuTuB | 2 | 0.178 CI95 [0.17 0.18] N = 5 |
0.482 CI95 [0.34 0.63] N = 5 |
0.003928 | −0.045 CI95 [−0.05 –0.04] N = 5 |
0.000027 | 0.373 CI95 [0.23 0.52] N = 5 |
0.002009 |
| R34H10 |
TuBua
(AOTU) |
5–6** | TuBu01 | 5 | 0.213 CI95 [0.20 0.23] N = 8 |
0.661 CI95 [0.61 0.71] N = 8 |
0.000000 | 0.033 CI95 [0.01 0.05] N = 8 |
0.003949 | 0.512 CI95 [0.46 0.57] N = 8 |
0.000000 |
|
TuBua
(BUa) |
5–6 | 0.200 CI95 [0.18 0.22] N = 7 |
0.728 CI95 [0.66 0.80] N = 7 |
0.000006 | 0.023 CI95 [0.01 0.04] N = 7 |
0.019318 | 0.512 CI95 [0.44 0.58] N = 7 |
0.000003 | |||
| R88A06 |
TuBus + TuBua
(AOTU) |
34 | TuBu01 TuBu06 TuBu08 TuBu09 |
5 4 10 11 |
0.188 CI95 [0.18 0.20] N = 11 |
0.485 CI95 [0.41 0.56] N = 11 |
0.000013 | ||||
|
TuBus
(BUs) |
28 | 0.221 CI95 [0.20 0.24] N = 6 |
0.587 CI95 [0.49 0.69] N = 6 |
0.000454 | −0.028 CI95 [−0.05 –0.01] N = 6 |
0.015342 | 0.305 CI95 [0.21 0.41] N = 6 |
0.000538 | |||
|
TuBua
(BUa) |
5–6 | 0.211 CI95 [0.20 0.23] N = 5 |
0.730 CI95 [0.62 0.84] N = 5 |
0.000230 | −0.022 CI95 [−0.04 –0.01] N = 5 |
0.017749 | 0.620 CI95 [0.51 0.73] N = 5 |
0.000086 | |||
| R49E09 |
TuBui
(AOTU) |
15–30 | TuBu02 TuBu03 TuBu05 |
7 15 5 |
0.162 CI95 [0.15 0.18] N = 5 |
0.154 CI95 [0.10 0.21] N = 5 |
0.758387 | ||||
|
TuBui
(BUi) |
15–30 | 0.085 CI95 [0.08 0.09] N = 12 |
0.132 CI95 [0.08 0.19] N = 12 |
0.088816 | |||||||
| R19C08 |
ER2
(BUs) |
~40*** | ER2_a ER2_b ER2_d ER2_c |
4 3 3 11 |
0.199 CI95 [0.16 0.24] N = 4 |
0.165 CI95 [0.09 0.24] N = 4 |
0.287547 | ||||
| R34D03 |
ER4m
(BUa) |
5 | ER4m | 5 | 0.219 CI95 [0.20 0.24] N = 25 |
0.506 CI95 [0.46 0.55] N = 25 |
0.000000 | −0.005 CI95 [−0.02 0.01] N = 25 |
0.579771 | 0.336 CI95 [0.29 0.38] N = 25 |
0.000000 |
|
ER4m
(EB) |
10 | 0.214 CI95 [0.19 0.24] N = 7 |
0.342 CI95 [0.24 0.44] N = 7 |
0.020818 | 0.028 CI95 [0.00 0.05] N = 7 |
0.039868 | 0.206 CI95 [0.11 0.31] N = 7 |
0.002423 | |||
| SS00096 |
E-PG
(PB) |
70 | EPG | 25 | 0.193 CI95 [0.19 0.20] N = 22 |
0.144 CI95 [0.12 0.17] N = 22 |
0.000133 | 0.008 CI95 [0.00 0.01] N = 22 |
0.000727 | 0.056 CI95 [0.03 0.08] N = 22 |
0.000062 |
†Data for Appendix 1–figure 1B.
*Unsorted group of MeTu-like neurons (partially traced: excludes dendrites in medulla).
**Also contains other TuBu neurons with weak expression, not visible in calcium imaging experiments.
***Also contains ER3w neurons (10 instances in hemibrain) and ER3p neurons (nine instances total of ER3p_a plus ER3p_b). A second, previously characterized, genetic driver for ER2 (R78B06) (Omoto et al., 2018) contains 20–25 cell bodies. Polarization-sensitive neurons in R19C08 likely correspond to ER2_a, ER2_b and ER2_d.