Skip to main content
. 2021 Mar 23;10:e63225. doi: 10.7554/eLife.63225

Appendix 2—table 1. Experimental genotypes.

Figure panel Genotype
Figure 1B w / w; R13E04-p65.AD / +; VT059781-Gal4.DBD / 10xUAS-mCD8::GFP
(R13E04-p65.AD; VT059781-Gal4.DBD is abbreviated as DmDRA1-split)
Figure 1B’ 10xUAS-mCD8::RFP, 13xLexAop-mCD8::GFP / w; R13E04-p65.AD / R24F06-LexA; VT059781-Gal4.DBD / +
Figure 1B’’ 10xUAS-mCD8::RFP, 13xLexAop-mCD8::GFP / w; Rh3/Rh4-Gal4 / R13E04-LexA; + / +
Figure 1C–H w / w; R13E04-p65.AD / UAS-sytGCaMP6s; VT059781-Gal4.DBD / UAS-tdTomato
Figure 1—figure supplement 1C-F w / w; Rh3/Rh4-Gal4 / UAS-sytGCaMP6s; + / UAS-tdTomato
Figure 1—figure supplement 1I w / w; R13E04-p65.AD / UAS-sytGCaMP6s; VT059781-Gal4.DBD / UAS-tdTomato
Figure 1—figure supplement 1J w / w; Rh3/Rh4-Gal4 / UAS-sytGCaMP6s; + / UAS-tdTomato
Figure 1—figure supplement 2 w / w; R13E04-p65.AD / UAS-sytGCaMP6s; VT059781-Gal4.DBD / UAS-tdTomato
Figure 2A w / w; + / +; R56F07-Gal4/10xUAS-mCD8::GFP
Figure 2B UAS-myr::GFP, QUAS-mtdTomato-3×HA / w; R13E04-p65.AD / trans-Tango; VT059781-Gal4.DBD / +
Figure 2C UAS-myr::GFP, QUAS-mtdTomato-3×HA / w; trans-Tango / +; Rh3/Rh4-Gal4 / +
Figure 2F,G Comparison between:
w / w; + / UAS-sytGCaMP6s; R56F07-Gal4 / UAS-tdTomato
w / w; + / UAS-sytGCaMP6s; R73C04-Gal4 / UAS-tdTomato
Figure 2—figure supplement 1A w / w; + / +; R56F07-Gal4 / 10xUAS-mCD8::GFP
Figure 2—figure supplement 1B-D MCFO / w; MCFO / +; R56F07-Gal4 / MCFO
Figure 3A,C,E w / w; + / UAS-sytGCaMP6s; R56F07-Gal4 / UAS-tdTomato
Figure 3B,D,F w / w; + / UAS-sytGCaMP6s; R73C04-Gal4 / UAS-tdTomato
Figure 3—figure supplement 1A,C,E w / w; + / UAS-sytGCaMP6s; R56F07-Gal4 / UAS-tdTomato
Figure 3—figure supplement 1B,D w / w; + / UAS-sytGCaMP6s; R73C04-Gal4 / UAS-tdTomato
Figure 4A–F w / w; + / UAS-sytGCaMP6s; R73C04-Gal4 / UAS-tdTomato
Figure 5A UAS-myr::GFP, QUAS-mtdTomato-3×HA / w; trans-Tango / +; R73C04-Gal4 / +
Figure 5B w / w; + / +; R17F12-Gal4 / 10xUAS-mCD8::GFP
Figure 5C–G w / w; + / UAS-sytGCaMP6s; R17F12-Gal4 / UAS-tdTomato
Figure 6B,C Comparison between:
w / w; + / UAS-opGCaMP6s; R49E09-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-opGCaMP6s; R88A06-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-opGCaMP6s; R34H10-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 6D,E w / w; + / UAS-opGCaMP6s; R49E09-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 6F,G w / w; + / UAS-opGCaMP6s; R88A06-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 6H,I w / w; + / UAS-opGCaMP6s; R34H10-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 7—figure supplement 1A,B Comparison between:
w / w; + / UAS-opGCaMP6s; R49E09-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-sytGCaMP6s; R49E09-Gal4 / UAS-tdTomato
w / w; + / UAS-opGCaMP6s; R88A06-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
w / w; + / UAS-opGCaMP6s; R34H10-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 7A w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
Figure 7B,C Comparison between:
w / w; + / UAS-sytGCaMP6s; R49E09-Gal4 / UAS-tdTomato
w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 7D w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
Figure 7E Comparison between:
w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 7F w / w; + / UAS-sytGCaMP6s; R88A06-Gal4 / UAS-tdTomato
Figure 7G w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 8B 10xUAS-mCD8::RFP, 13xLexAop-mCD8::GFP / w; R34D03-LexA / +; R34H10-Gal4 / +
Figure 8C w / w; + / UAS-opGCaMP6s; R19C08-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 8D Comparison between:
w / w; + / UAS-opGCaMP6s; R19C08-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 8E w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 8F w / w; R34D03-LexA / UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11; R34H10-Gal4 / +
Figure 8G w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 8H w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 8—figure supplement 1A,C,E w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 8—figure supplement 1B,D,F w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 9A–E w / w; + / UAS-sytGCaMP6s; R34D03-Gal4 / UAS-tdTomato
Figure 9F w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
Figure 9G w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
Figure 10B,C w / w; R19G02-p65.AD / UAS-sytGCaMP6s; R70G12-Gal4.DBD / UAS-tdTomato
(R19G02-p65.AD; R70G12-Gal4.DBD is abbreviated as SS00096)
Figure 10D,E Comparison between:
w / w; R19G02-p65.AD / UAS-sytGCaMP6s; R70G12-Gal4.DBD / UAS-tdTomato
w / w; + / UAS-sytGCaMP6s; R34D03-Gal4 / UAS-tdTomato
Figure 10F–I w / w; R19G02-p65.AD / UAS-sytGCaMP6s; R70G12-Gal4.DBD / UAS-tdTomato
Figure 10—figure supplement 1A w / w; R19G02-p65.AD / +; R70G12-Gal4.DBD / 10xUAS-mCD8::GFP
Figure 10—figure supplement 1B w / w; R19G02-p65.AD / R34D03-LexA; R70G12-Gal4.DBD / UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11
Figure 10—figure supplement 1C Comparison between:
w / w; + / UAS-sytGCaMP6s; R34H10-Gal4 / UAS-tdTomato
w / w; + / UAS-opGCaMP6s; R34D03-Gal4 / UAS-opGCaMP6s, UAS-CD4::tdTomato
w / w; R19G02-p65.AD / UAS-sytGCaMP6s; R70G12-Gal4.DBD / UAS-tdTomato
Figure 10—figure supplement 1E-G w / w; R19G02-p65.AD / UAS-sytGCaMP6s; R70G12-Gal4.DBD / UAS-tdTomato