Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | Rh3/Rh4-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0058802;RRID:BDSC_8603 | Synonym: pan-R7-Gal4 Wernet et al., 2006 |
| Genetic reagent (D. melanogaster) | DmDRA1 split (R13E04-p65.AD; VT059781-Gal4.DBD) | This paper | FLYB:FBti0187820;FLYB:FBti0194675 | Available upon request. Components: Dionne et al., 2018; Tirian and Dickson, 2017 |
| Genetic reagent (D. melanogaster) | R13E04-LexA | Bloomington Drosophila Stock Center | FLYB:FBtp0087918;RRID:BDSC_52457 | Pfeiffer et al., 2013 |
| Genetic reagent (D. melanogaster) | R24F06-LexA | Bloomington Drosophila Stock Center | FLYB:FBti0155501;RRID:BDSC_52695 | Pfeiffer et al., 2013 |
| Genetic reagent (D. melanogaster) | R56F07-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0136983;RRID:BDSC_39160 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R73C04-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0138074;RRID:BDSC_39815 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R17F12-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0133538;RRID:BDSC_48779 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R34H10-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0135093;RRID:BDSC_49808 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R88A06-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0139280;RRID:BDSC_46847 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R49E09-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0136346;RRID:BDSC_38692 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R34D03-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0135043;RRID:BDSC_49784 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | R19C08-Gal4 | Bloomington Drosophila Stock Center | FLYB:FBti0133678;RRID:BDSC_48845 | Jenett et al., 2012 |
| Genetic reagent (D. melanogaster) | SS00096 (R19G02-p65.AD; R70G12- Gal4.DBD) |
other | FLYB:FBtp0117182;FLYB:FBtp0122173 | Kim et al., 2017 Provided by V. Jayaraman and T.Wolff, available via https://www.janelia.org/split-GAL4 |
| Genetic reagent (D. melanogaster) | R60D05-LexA | Bloomington Drosophila Stock Center | FLYB:FBti0156166;RRID:BDSC_52867 | Pfeiffer et al., 2013 |
| Genetic reagent (D. melanogaster) | R34D03-LexA | Bloomington Drosophila Stock Center | FLYB:FBti0135043;RRID:BDSC_49784 | Pfeiffer et al., 2013 |
| Genetic reagent (D. melanogaster) | 20×UAS-IVS-Syn21-opGCaMP6s-p10 | other | FLYB:FBti0195669 | Provided by G. Rubin |
| Genetic reagent (D. melanogaster) | UAS-mCD4::tdTomato | Bloomington Drosophila Stock Center | FLYB:FBtp0068010;RRID:BDSC_35837 | Han et al., 2011 |
| Genetic reagent (D. melanogaster) | UAS-sytGCaMP6s | Bloomington Drosophila Stock Center | FLYB:FBti0181795;RRID:BDSC_64415 | Cohn et al., 2015 |
| Genetic reagent (D. melanogaster) | UAS-tdTomato | Bloomington Drosophila Stock Center | FLYB:FBti0145104;RRID:BDSC_36328 | Schulte, 2011; Shaner et al., 2004 |
| Genetic reagent (D. melanogaster) | 10×UAS-mCD8::GFP | Bloomington Drosophila Stock Center | FLYB:FBti0131930;RRID:BDSC_32184 | Pfeiffer, 2010 |
| Genetic reagent (D. melanogaster) | 26×LexAop-mCD8::GFP | Bloomington Drosophila Stock Center | FLYB:FBti0131946;RRID:BDSC_32207 | Pfeiffer, 2010 |
| Genetic reagent (D. melanogaster) | 10×UAS-mCD8::RFP; 13×LexAop-mCD8::GFP | Bloomington Drosophila Stock Center | FLYB:FBst0032229;RRID:BDSC_32229 | Pfeiffer, 2010 |
| Genetic reagent (D. melanogaster) | UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11 | Bloomington Drosophila Stock Center | FLYB:FBtp0111281;RRID:BDSC_64314 | Components: Gallio, 2016; Macpherson et al., 2015 |
| Genetic reagent (D. melanogaster) | MCFO-4 | Bloomington Drosophila Stock Center | FLYB:FBst0064088;RRID:BDSC_64088 | Nern et al., 2015 |
| Genetic reagent (D. melanogaster) | MCFO-5 | Bloomington Drosophila Stock Center | FLYB:FBst0064089;RRID:BDSC_64089 | Nern et al., 2015 |
| Genetic reagent (D. melanogaster) | MCFO-6 | Bloomington Drosophila Stock Center | FLYB:FBst0064090;RRID:BDSC_64090 | Nern et al., 2015 |
| Genetic reagent (D. melanogaster) | GRASP | Bloomington Drosophila Stock Center | FLYB:FBst0064314;RRID:BDSC_64314 | Macpherson et al., 2015 |
| Genetic reagent (D. melanogaster) | trans-Tango | Bloomington Drosophila Stock Center | FLYB:FBst0077124;RRID:BDSC_77124 | Talay et al., 2017 Provided by G. Barnea |
| Antibody | Anti-DN-cadherin (rat monoclonal) | Developmental Studies Hybridoma Bank | Cat#:DN-Ex #8; RRID:AB_528121 |
(1:20) |
| Antibody | Anti-Neuroglian (mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat#:BP 104; RRID:AB_528402 |
(1:30) |
| Antibody | Anti-GFP (chicken polyclonal) | Abcam | Cat#:ab13970; RRID:AB_300798 |
(1:1000) |
| Antibody | Anti-DsRed (rabbit polyclonal) | Takara Bio | Cat#:632496; RRID:AB_10013483 |
(1:1000) |
| Antibody | Anti-HA (rabbit monoclonal) | Cell Signaling Technology | Cat#: 3724S; RRID:AB_1549585 |
(1:300) |
| Antibody | Anti-V5 (mouse monoclonal) | Thermo Fisher Scientific | Cat#:37–7500; RRID:AB_2533339 |
(1:1000) |
| Antibody | Anti-Mouse conjugated to Cy5 (goat polyclonal) | Jackson ImmunoResearch | Cat#:115-175-166; RRID:AB_2338714 |
(1:300) |
| Antibody | Anti-Rat conjugated to Cy3 (goat polyclonal) | Jackson ImmunoResearch | Cat#:112-165-167;RRID:AB_2338251 | (1:300) |
| Antibody | Anti-Rat conjugated to Cy5 (goat polyclonal) | Jackson ImmunoResearch | Cat#:112-175-143;RRID:AB_2338263 | (1:300) |
| Antibody | Anti-Rabbit conjugated to Cy3 (goat polyclonal) | Jackson ImmunoResearch | Cat#:111-165-003;RRID:AB_2338000 | (1:300) |
| Antibody | Anti-GFP conjugated to Alexa488 (rabbit polyclonal) | Thermo Fisher Scientific | Cat#:A-21311;RRID:AB_221477 | (1:1000) |
| Antibody | Anti-GFP (mouse monoclonal) | Sigma-Aldrich | Cat#:G6539; RRID:AB_259941 |
(1:1000) |
| Antibody | Anti-Chicken conjugated to Alexa488 (goat polyclonal) | Thermo Fisher Scientific | Cat#:A-11039;RRID:AB_2534096 | (1:1000) |
| Antibody | Anti-Mouse conjugated to Alexa488 (goat polyclonal) | Thermo Fisher Scientific | Cat#:A-10680;RRID:AB_2534062 | (1:1000) |
| Software, algorithm | Fiji | ImageJ | RRID:SCR_002285 | Schindelin et al., 2012 |
| Software, algorithm | SlideBook 6 | Intelligent Imaging Innovations | RRID:SCR_014300 | |
| Software, algorithm | MATLAB R2017a | Mathworks | RRID:SCR_001622 |