Figure 6.

LXA4’s protective effect on LPS-induced lung injury was abrogated by BOC-2. Mice were intraperitoneally injected with BOC-2 (50 μg/kg) 2 h before LPS (1 mg/kg) administration with or without of LXA4 (0.1 µg/mouse). The beneficial effects of LXA4 were abrogated by BOC-2, as assessed by histological analysis (A), acute lung injury score (B), CCL2 (C), CXCL2 (E), MMP-9 (G), TNF-α (H) and IL-1β (I) protein expression, and the number of neutrophils (J) in the BALF. Resident macrophages were sorted and pretreated with BOC-2 (10 μM) 30 min before cultured with LPS (1 μg/mL) in the presence or absence of LXA4 (100 nM) for 24 h. CCL2 (D) and CXCL2 (F) mRNA level were measured by RT-PCR. The data are presented as the mean ± SEM, n =6- 9. *p<0.05, **p<0.01.