Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 12;16:2803–2818. doi: 10.2147/IJN.S284560

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Wang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Downregulation of hsa_circ_00074854 inhibited the migration, invasion, and EMT of HepG2 cells via HuR. (A) Relative HuR mRNA levels were detected by RT-qPCR in HepG2 cells with hsa_circ_00074854 knockdown. (B) Western blot analysis for HuR protein levels in HepG2 cells transfected with hsa_circ_00074854 shRNA1. **P < 0.01 vs shRNA-NC group. (C) RT-qPCR analysis of hsa_circ_00074854 expression in HepG2 cells transfected with lenti-hsa_circ_00074854. **P < 0.01 vs lenti-NC group. (D) HepG2 cells with hsa_circ_00074854 overexpression or knockdown were treated with 50 μg/mL CHX for 12 h. Western blot assay was used to detect the expression of HuR in HepG2 cells. **P < 0.01. (E) RT-qPCR analysis of HuR expression in HepG2 cells transfected with HuR shRNA1, HuR shRNA2 or lenti-HuR. *P < 0.05; **P < 0.01. (F) The migratory ability of HepG2 cells transfected with HuR shRNA1 detected by transwell migration assay. **P < 0.01 vs shRNA-NC group. (G) The invasive ability of HepG2 cells transfected with HuR shRNA1 detected by transwell invasion assay. **P < 0.01 vs shRNA-NC group. (H) Western blot analysis of ZEB-1, E-cadherin and vimentin in HepG2 cells transfected with HuR shRNA1. **P < 0.01 vs shRNA-NC group. (I) Western blot analysis of ZEB-1, E-cadherin and vimentin in HepG2 cells transfected with hsa_circ_00074854 shRNA1 or hsa_circ_00074854 shRNA1 plus lenti-HuR. **P < 0.01 vs NC group; ^##P < 0.01 vs circRNA-shRNA1 group. (J) Flow cytometry assay was used to determine cell apoptosis. **P < 0.01 vs NC group; ^##P < 0.01 vs circRNA-shRNA1 group.

Abbreviations: CHX, cycloheximide; circRNA shRNA1, HepG2 cells transfected with hsa_circ_00074854-shRNA1; lenti-circRNA, HepG2 cells transfected with lenti-hsa_circ_00074854; shRNA-NC, HepG2 cells transfected with shRNA negative control.