Figure 8.

Exosomes with downregulated hsa_circ_00074854 inhibited macrophage M2 polarization to suppress migration, invasion and EMT in HepG2 cells in vitro. (A) Schema for representing the experiment procedures. (B) The migration and invasion abilities of HepG2 cells co-cultured with macrophages treated with exosomes were assessed by the transwell co-culture system. (C) HepG2 cells incubated with the supernatants of macrophages treated with exosomes. Western blot analysis of ZEB-1, E-cadherin and vimentin in HepG2 cells. **P < 0.01 vs HepG2 + M group; ^##P < 0.01 compared with HepG2 + M^Exo group.

Abbreviations: M, macrophages (PMA-differentiated human THP-1 monocytes); M^Exo, macrophages co-cultured with exosomes derived from HepG2 cells; M^{Exo-circRNA shRNA1}, macrophages co-cultured with exosomes derived from hsa_circ_00074854 shRNA1-transfected HepG2 cells; M^IL4/IL13, macrophages co-cultured with IL4 and IL13 (20 ng/mL); PMA, phorbol-12 myristate-13 acetate.