REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GLIS3	Yang et al. (2011); L. Chan lab, Baylor College of Medicine, Texas, USA	PMID 21786021
H3 mono methyl K4	Abcam	ab8895
Histone H3 (acetyl K27)	Abcam	ab4729
H3 tri-methyl K4	Millipore	04-745
FLAG M2 (against GLIS3-FLAG)	Sigma FLAG-M2	F3165
PDX1	BCBC	Ab2027
Insulin	Dako	A0564
gunie pig anti-Glucagon	MilliporeSigma	4031-01F
rabbit anti glucagon	Dako	A0565
Somatostatin	Dako	A0566
GFP	R&D Systems	AF4240
Immunoflourescence secondary antibodies	Jackson immunoResearch	Cy3, Cy5 and Alexa flour 488
Biological samples
Human Islets	European Consortium on Islet Transplantation (ECIT) AND Islets for Basic Research Program supported by the Juvenile Diabetes Research Foundation	https://ecit.dri-sanraffaele.org and JDRF-program 2-RSC-2019-724-I-X
Chemicals, peptides, and recombinant proteins
Antibody diluent	DAKO	S3022
β-2-mercaptoethanol	Sigma-Aldrich	M3148-25ML
BSA	Sigma-Aldrich	A3059
BSA (for Endo-C culture only)	Roche	10775835001
Calf Serum	Sigma-Aldrich	C8056-100ML
Collagenase	Roche	11215809103
Diethylaminoethyl (DEAE)	Sigma-Aldrich	67578-5G
DMEM	Lonza	be12-604f
DMEM 5.5mM Glucose	Thermofisher Scientific	31885023
dsDNA BR assay kit	Thermofisher Scientific	Q32853
dsDNA HS assay kit	Thermofisher Scientific	Q32854
ECM gel	Sigma-Aldrich	E1270-10ML
EDTA	Thermofisher Scientific	AM9260G
Ethanol (immunoflourescence)	Panreac Applichem, Spain	141086.1211
Ethanol	Merck Millipore	108543
Fetal Calf Serum (FBS)	Cambrex	14-801/ 91s1810-500
Fibronectin	Sigma-Aldrich	F1141
Formaldehyde	Calbiochem	344198
Glucose	Sigma-Aldrich	G-8270-1KG
Glycine	Sigma-Aldrich	50046-250G
HBSS	Invitrogen	14060-040
HEPES	Sigma-Aldrich	H7523-50G
Histopaque 1077	Sigma-Aldrich	10771-500ml
Histopaque 1119	Sigma-Aldrich	11191-100ml
L-Glutamine	Cambrex	BE17-605E
LiCl	Sigma-Aldrich	L9650-100G
Lipofectamine 2000	Invitrogen	11668-019
NEBNext Ultra DNA Library Prep Kit	New England Biolabs	E7370L
Newborn calf serum (Endo-C during passage)	GIBCO	16010-159
Nicotinamide	Sigma-aldrich	481907
Normal donkey serum	Jackson Immunoresearch	017-000-121
NP-40 substitute IGEPAL CA-630	Sigma-Aldrich	I8896
Paraformaldehyde	Agar Scientific	R1026
PBS	Sigma-Aldrich	D-8537
Penicillin	Lonza	DE17-602E/ 17-745E
Phenol chloroform	Sigma-Aldrich	77617-100ML
Power SYBR green mastermix	Applied Biosystems	4368702
Protease inhibitor cocktail	Roche	4693132001
Protein A Sepharose beads	Ge Healthcare	17-0780-01
Protein G Sepharose beads	Ge Healthcare	17-0618-01
Proteinase K	Fermentas	EO0492
Qiaquick columns	QIAGEN	28106
RNase A	Sigma-Aldrich	R4875-100MG
RPMI 1640 medium	Lonza	BE12-702F
RPMI 1640 medium- without glucose	Lonza	BE12-752F
SDS	Sigma-Aldrich	71736-500ML
SDS (ChIP)	Invitrogen	15553-027
Sodium selenite	Sigma-Aldrich	S9133
Sodium-chloride	Sigma-Aldrich	S3014
Sodium-deoxycholate	Sigma-Aldrich	30970-100G
Stratagene Site directed mutagenesis kit	Stratagene (currently Thermofisher)	A13282
Streptomycin	Lonza	DE17-602E/ 17-745E
Superscript III Reverse Transcriptase	Invitrogen	18080093
SYBR green mastermix	Applied Biosystems	4368708
TaqMan Probes	Applied Biosystems	costom
Transferrin	Sigma-Aldrich	T8158-100MG
TriPure reagent	Invitrogen/ Roche	11667165001
Tris-HCL	Life Technologies	15568-025
Trypsin-EDTA 0.05%	GIBCO	25300062
Xylene	PanReac/AppliChem	211769.1714
Critical commercial assays
Dual-LuciferaseReporter Assay System	Promega	E1960
Deposited data
GLIS3 ChIP-seq in human islets	This paper	GSE151405
H3K4me1, Human fetal pancrease	Cebola et al., 2015	PMID: 25915126
H3K4me3, Human fetal pancrease	Cebola et al., 2015	PMID:25915126
H3K4me1, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
H3K4me3, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
H3K27ac, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
PDX1, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
NKX6-1, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
FOXA2, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
MAFB, Human donor-derived islet	Pasquali et al., 2014	PMID:24413736
RNA-seq, Human donor-derived islet	Akerman et al., 2017	PMID:28041957
Experimental models: cell lines
EndoCβ-H1	Philippe Ravassard Laboratory	N/A
Mouse embryonic fibroblasts (MEFs)	Generated in this study	N/A
HEK293T	ATCC	ATCC CRL-11268
MCF7	ATCC	HTB-22
SW480	ATCC	CCL-228
Experimental models: organisms/strains
C57BL/6	Charles River	C57BL/6 (inbred)
HIPKI, HIPKI-C331G	Generated in this study	N/A
Oligonucleotides
Primers used in this study - Table S3	IDT	N/A
Taqman Probe Ins1	Applied Biosystems	Mm01259683_g1
Taqman Probe Ins2	Applied Biosystems	Mm00731595_gh
Taqman Probe INS	Applied Biosystems	Hs02741908_m1
Taqman Probe Actb	Applied Biosystems	Mm00607939_s1
Taqman Probe ACTB	Applied Biosystems	Hs01060665_g1
Software and algorithms
FASTQC	Babraham institute	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
MACS2	Zhang et al., 2008	https://github.com/macs3-project/MACS/wiki/Install-macs2
Picard Suite	Broad Institute	https://broadinstitute.github.io/picard/
Rstudio	RStudio Team (2020). RStudio: Integrated Development for R.	https://www.rstudio.com/