Fig. 6. Hierarchal regulation of SUV39H2 in TS cells.
A) Validation of Cdx2 shRNA knockdown of Cdx2 transcript by RT-qPCR. B) Effects of Cdx2 shRNA knockdown on Suv39h2 transcript by RT-qPCR. C) Schematic layout of the rat Suv39h2 gene and the location of one of the regions (No. 1: 4369 to −4200 bp upstream of the transcription start site) surveyed in control and CDX2 knockdown TS cells by ChIP analysis with IgG or antibodies specific to CDX2. Asterisks indicate significant differences between control and CDX2 knockdown cells, *P<0.01.