Extended Data Fig. 1 |.
Low levels of bacteria are detected after topical application of bacteria on mice with inflamed skin. Colony forming units (CFU) of S. aureus and CoNS were measured from the spleen of OVA-sensitized FLGft/ft Balb/c mice that were colonized by S. aureus for 4 days and treated by ShA9 for 3 days. Spleens (50–100 mg) were surgically excised and homogenized in 1 mL PBS. Live S. aureus and CoNS CFU were counted on mannitol salt agar with egg yolk and CFU counts adjusted based on wet weight of the excised spleens. Data represent mean ± SEM of biological replicates in individual mouse (n = 8). No statistical difference was detected by two-tailed unpaired parametric t-test.