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. Author manuscript; available in PMC: 2022 Apr 1.

Published in final edited form as: Biochem Pharmacol. 2021 Feb 10;186:114462. doi: 10.1016/j.bcp.2021.114462

Figure 3. — Flavones, flavopiridol, and atuveciclib inhibit provirus expression in the simultaneous presence of LRAs. A, Representative examples of GFP expression in J-Lat 9.2 cells treated with 0.1% DMSO vehicle control, 10 μM prostratin, or prostratin plus 10 μM apigenin. Values indicate percent GFP-positive cells. (B-D) Effect of compounds after 24 hours’ incubation on LRA-induced provirus expression in J-Lat 9.2 cells. Cells were treated with 10 ng/mL TNFα (B), 0.1 μM panobinostat (C), or 10 μM prostratin (D) in the concurrent presence of test compounds at defined concentrations. Horizontal dotted lines denote average % GFP expression in cells treated with LRA only. E, Representative examples of GFP expression in J-Lat 10.6 cells treated with 0.1% DMSO, 10 μM JQ1, or JQ1 plus 10 μM apigenin. Values indicate percent GFP-positive cells. (F-H) Effect of compounds after 24 hours’ incubation on LRA-induced provirus expression in J-Lat 10.6 cells. Cells were treated with 10 ng/mL TNFα (F), 10 μM JQ1 (G), or 10 mM HMBA (H) in the concurrent presence of test compounds at defined concentrations. Red dotted lines denote percent spontaneous GFP expression in J-Lat 10.6 cells treated with 0.1% DMSO, and black dotted lines denote percent GFP expression in the presence of LRA only. I, Representative examples of viral p24^Gag protein expression in OM-10.1 cells treated with 0.1% DMSO, 1 ng/mL TNFα, or TNFα plus 10 μM apigenin. Values indicate percent p24^Gag-positive cells. (J) Effect of compounds after 24 hours’ incubation on concurrent 1 ng/mL TNFα-induced provirus expression in OM-10.1 cells. Black dotted line denotes p24^Gag production in the presence of TNFα only. (K-L) Effects of compounds after 24 hours’ incubation on Jurkat (K) and OM10.1 (L) cell viability, as measured using Viacount dye. In K-L, data are presented relative to viability of 0.1% DMSO vehicle control-treated cells (black dotted lines).

Figure 3. — Flavones, flavopiridol, and atuveciclib inhibit provirus expression in the simultaneous presence of LRAs. A, Representative examples of GFP expression in J-Lat 9.2 cells treated with 0.1% DMSO vehicle control, 10 μM prostratin, or prostratin plus 10 μM apigenin. Values indicate percent GFP-positive cells. (B-D) Effect of compounds after 24 hours’ incubation on LRA-induced provirus expression in J-Lat 9.2 cells. Cells were treated with 10 ng/mL TNFα (B), 0.1 μM panobinostat (C), or 10 μM prostratin (D) in the concurrent presence of test compounds at defined concentrations. Horizontal dotted lines denote average % GFP expression in cells treated with LRA only. E, Representative examples of GFP expression in J-Lat 10.6 cells treated with 0.1% DMSO, 10 μM JQ1, or JQ1 plus 10 μM apigenin. Values indicate percent GFP-positive cells. (F-H) Effect of compounds after 24 hours’ incubation on LRA-induced provirus expression in J-Lat 10.6 cells. Cells were treated with 10 ng/mL TNFα (F), 10 μM JQ1 (G), or 10 mM HMBA (H) in the concurrent presence of test compounds at defined concentrations. Red dotted lines denote percent spontaneous GFP expression in J-Lat 10.6 cells treated with 0.1% DMSO, and black dotted lines denote percent GFP expression in the presence of LRA only. I, Representative examples of viral p24^Gag protein expression in OM-10.1 cells treated with 0.1% DMSO, 1 ng/mL TNFα, or TNFα plus 10 μM apigenin. Values indicate percent p24^Gag-positive cells. (J) Effect of compounds after 24 hours’ incubation on concurrent 1 ng/mL TNFα-induced provirus expression in OM-10.1 cells. Black dotted line denotes p24^Gag production in the presence of TNFα only. (K-L) Effects of compounds after 24 hours’ incubation on Jurkat (K) and OM10.1 (L) cell viability, as measured using Viacount dye. In K-L, data are presented relative to viability of 0.1% DMSO vehicle control-treated cells (black dotted lines).