Figure 6.

Compound inhibition of HDACs inversely correlates with reinforced HIV latency following culture withdrawal. (A) Examples of cellular HDAC activity in the absence or presence of 0.1 μM panobinostat, as measured using the HDAC-Glo I/II assay. (B) Effects of compounds after 90 minutes’ incubation on Jurkat cell HDAC activity. (C-D) GFP expression in J-Lat 10.6 cells treated with compounds or 0.1% DMSO for 21 days (pre-wash), then washed extensively and incubated for an additional 24 hours (C) or up to 14 days (D) in the absence of compounds (post-wash). Data are presented relative to cells treated with 0.1% DMSO. Horizontal dotted lines in B-D denote activities at 100 and 50% of cells treated with 0.1% DMSO.