Figure 1.

Oligoribonucleotide radical biotinylation is catalyzed by HRP in the presence of H₂O₂ and biotin tyramide. Biotinylation is detected by a streptavidin‐dependent gel shift under denaturing conditions, followed by staining with SYBR Gold. A) Biotinylation of LJM‐6133 containing G₃ motifs. B) Biotinylation of LJM‐6135 lacking G₃ motifs. C) Biotinylation of LJM‐6247 containing the same number of G residues as LJM‐6133, without G₃ motifs. Statistics were calculated from independent experiments performed in triplicate; S.D.: standard deviation.