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Oligodeoxyribonucleotide 5’‐FAM modification is necessary and sufficient for efficient radical biotinylation catalyzed by HRP in the presence of H2O2 and biotin tyramide. Biotinylation of the indicated DNA oligonucleotides is detected by a streptavidin‐dependent gel mobility shift under native conditions. Statistics are calculated from independent experiments performed in triplicate; S.D. is standard deviation.
