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. 2021 Jan 27;22(8):1400–1404. doi: 10.1002/cbic.202000854

Figure 3.

Figure 3

Biotinylation confirmed by free biotin competition for streptavidin binding to LJM‐6132. Biotin excess of 0–200 μM (lanes 3–8) in a 30‐min pre‐incubation inhibits LJM‐6132 binding by streptavidin under native gel conditions.