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. Author manuscript; available in PMC: 2022 Apr 15.
Published in final edited form as: Mol Cell. 2021 Feb 24;81(8):1789–1801.e5. doi: 10.1016/j.molcel.2021.01.040

Figure 1. tNET-Structureseq mapping of nascent RNA structure.

Figure 1.

A. Enzymatic (tNet-RNAse-seq ) and DMS (tNet-MaPseq ) probing of nascent pol II transcripts.

B. Distribution of tNet-RNAse-seq and tNet-MaPseq compared to mRNAseq reads in HEK293 WT Amr pol II cells.

C. Metaplot of putative exon junction complex footprints (RNAse I+VI resistant, blue arrow) upstream of splice sites (SS).

D. Predicted structures with DMS reactivities of the non-canonical XBP1 intron (arrows) (Yoshida et al., 2001) (chr22:29,192,089–29,192,172) and XIST exon 4 (chrX :73,050,979–7,3051,093) within nascent RNA.

E. Nascent RNA structure across an intron (chr16:89,867,703–89,871,732). Normalized DMS reactivity, RNAse I, RNAse VI and RNAse I+VI resistant reads, fraction of A-I editing per base and nascent RNA seq reads (tNET-seq) are shown.