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. Author manuscript; available in PMC: 2022 Apr 15.
Published in final edited form as: Mol Cell. 2021 Feb 26;81(8):1816–1829.e5. doi: 10.1016/j.molcel.2021.02.004

Figure 1. Deletions of recombination or checkpoint genes decrease ALT frequency.

Figure 1.

(A) ALT survivors form in every tlc1Δ culture when large populations (105 cells/ml) are passaged in liquid cultures (dissection and passaging scheme above).

(B) ALT survivors form rarely when small populations (250 cells/ml) are passaged allowing use of Poisson statistics for ALT frequency calculation.

(C) Effect of deletions of recombination, checkpoint, and telomere capping genes on the frequency of ALT in tlc1Δ cells. tlc1Δ Liquid was calculated as described in (B). For all other cultures the modified passaging scheme with plating cells after second passage was used. Medians of ALT frequencies and 95% confidence intervals (in parenthesis) are shown. The arrows and folds change on the top represent reduction (or increase) of ALT frequency as compared to tlc1Δ.

(See also Figure S1, S2 and Tables S1, S2)