| Plasmids |
| pJH132 |
James Haber |
Wu & Haber 1995 |
| Chemicals |
|
|
| Zymolase |
US biological Co. |
NC0439194 |
| Phenol/Chloroform/Isoamyl alcohol |
Millipore Sigma |
P3803-400ML |
| Sorbitol |
Fisher |
BP439-500 |
| Isopropanol |
Fisher |
BP2618500 |
| Potassium acetate |
Sigma Aldrich |
P1190-500G |
|
XhoI |
New England Biolabs |
R0146S |
| AMPure beads |
Beckman Coulter |
A63880 |
| Deposited Data |
|
|
| Raw ONT sequencing data |
This Paper |
PRJNA630168 |
| Raw PacBio sequencing data |
This Paper |
PRJNA630168 |
| Uncropped Photographs |
This Paper |
http://dx.doi.org/10.17632/m6xzygyvjt.1 |
| Critical Commercial Assays |
|
|
| QX200 Evagreen Supermix |
BioRad |
186-4034 |
| QX200 Evagreen Droplet Oil |
BioRad |
186-4033 |
| NEBNext FFPE DNA repair Kit |
New England Biolabs |
M660S |
| NEBNext Quick T4 DNA ligase |
New England Biolabs |
E6056S |
| MinIon Platform |
Oxford Nanopore Technologies |
MIN101B |
| MinIon Flow Cell |
Oxford Nanopore Technologies |
R9.4.1 |
| MinIon Sample Prep Kit |
Oxford Nanopore Technologies |
SQK-LSK109 |
| Experimental Models: Organisms/Strains |
|
|
| Yeast Saccharomyces cerevisiae: AM939 |
James Haber |
Malkova et al. 2004 |
| Oligonucleotides |
| Yeast Y’ probe FWD: CGCGAATTCGCCCTACAGCACTTCTACATAGC |
This Paper |
OL3467 |
| Yeast Y’ probe RVS: CGAGAATTCCAGCGTTTGCGTTCCATGACG |
This Paper |
OL3468 |
| Software and Algorithms |
|
|
| CLCgenomics12 |
Qiagen |
https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/analysis-and-visualization/qiagen-clc-genomics-workbench/ |
| MinKnow |
Oxford Nanopore Technology |
https://nanoporetech.com/ |
| ONT guppy |
Oxford Nanopore Technology |
https://nanoporetech.com/ |
| Graphpad Prism 8 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
