Skip to main content
. 2021 Apr 16;12:2299. doi: 10.1038/s41467-021-22620-3

Fig. 3. TREM2 and Mincle preferentially recognize distinct MA-containing lipids based on their glycosylation.

Fig. 3

a Chemical structure of glycosylated (TDM and GMM) and non-glycosylated (GroMM and fMA) MA-containing lipids found in Mtb. The structures of α-mycolate-containing lipids are included. b The indicated amounts of TREM2 or Mincle-Fc fusion proteins were added to wells coated with 0.5 μg/well TDM, GMM, GroMM, or fMA, followed by incubation with anti-human IgG-horseradish peroxidase and detection of bound proteins using an enzyme-linked immunosorbent assay (ELISA)-based colorimetric assay. c NFAT-GFP reporter cells expressing TREM2 + DAP12 or Mincle + FcRγ were stimulated with the indicated amounts of TDM, GMM, GroMM, or fMA coated on the plates for 24 h, followed by analysis of GFP fluorescence by flow cytometry. Values are plotted as percent maximal response of the largest value. Data are presented as mean ± SEM of duplicate assays and are representative of at least three independent experiments. Source data are provided as a Source data file.