Fig. 4. Distinct lipid recognition and macrophage activation through Mincle and TREM2.

a–c Peritoneal macrophages from wild-type (WT), Trem2^−/−, or Clec4e^−/− mice (a), WT, Tyrobp^−/−, or Fcer1g^−/− mice (b), or WT or Card9^−/− mice (c) were stimulated with the indicated amounts of TDM, GMM, GroMM, or fMA coated on the plates or with LPS (100 ng/ml) for 24 h. WT cells were stimulated in the presence or absence of the Syk inhibitor BAY-613606 (BAY) (c). MCP-1 and TNF production in the culture supernatant was measured by ELISA. Data are presented as the mean ± SEM of triplicate assays and are representative of three independent experiments. The statistical significance was calculated by two-way ANOVA followed by Bonferroni’s test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.