Fig. 7. TREM2 deficiency accelerates the clearance of mycobacterial infection.

a, b WT or Trem2^−/− BMDMs were infected in vitro with M. bovis BCG (MOI 10) for 4 h. After washing, the cells were further cultured for 24 h, and nitrite concentration in the culture supernatant was measured by Griess assay (a). The cells were collected, lysed, and CFUs of M. bovis BCG were counted (b). The statistical significance was calculated by two-tailed unpaired t test. c–f WT or Trem2 ^−/− mice (day 3, n = 5; day 14, n = 7) were infected intratracheally with 7.5 × 10⁶ CFU of M. bovis BCG. The lung tissues were collected at days 3 and 14 after infection, homogenized, and CFU were determined (c). The expressions of Ccl2, Adgre1, and Nos2 in the lungs from uninfected control (n = 5) and infected mice were analyzed by qRT-PCR (d–f). Data are presented as the mean ± SEM and are representative of two independent experiments. The statistical significance was calculated by two-tailed unpaired t test. *p < 0.05, **p < 0.01. Source data are provided as a Source data file.