Table 2.

DNA sample characteristics and the gene alterations detected by next-generation sequencing.

Pt #	Tumor samplesa and cfDNA (days before death)		Histology	DNA conc. (ng/mL)	Sequence depthb	Number of gene alterations	(Both in tumor and cfDNA)c	(cfDNA alone)d	VAFs of active EGFR mutation (%)
1	Lung	Primary	Squamous carcinoma	1.97	1031	891	9	110	56.9
	Adrenal	Metastasis	Adenocarcinoma	1.43	1110	1235	17	102	82.2
	Soft tissue	Metastasis	Carcinoma	15.4	1431	129	11	108	8.7
	cfDNA	85		5.9	9151	119			27
2	Lung	Primary	Small cell carcinoma	10.4	2380	141	55	119	79.7
	Lung	Metastasis	Adenocarcinoma	5.18	4026	130	78	96	80.1
	Lymph node	Metastasis	Small cell carcinoma	102	3887	155	48	126	19.8
	Liver	Metastasis	Adenocarcinoma	32.8	3708	110	71	103	–
	cfDNA	1		4.4	2487	174			4.7
3	Lung	Primary	Adenocarcinoma	173	3846	97	34	53	40
	Liver	Metastasis	Adenocarcinoma	163	3412	83	29	58	35.2
	Renal	Metastasis	Adenocarcinoma	20.6	3514	140	43	44	–
	cfDNA	0		2.5	8009	87			3.4
4	Lung	Primary	Adenocarcinoma	96.2	2217	201	139	59	55.2
	Lymph node	Metastasis	Adenocarcinoma	84.6	3829	223	135	63	47.3
	cfDNA	21		1.56	8484	198			47.7
5	Lung	Primary	Adenocarcinoma	15.7	1050	68	27	51	–
	Lymph node	Metastasis	Adenocarcinoma	8.12	679	60	21	57	6.8
	cfDNA	68		6.76	1193	78			–
6	Lung	Primary	Adenocarcinoma	171	865	185	95	267	48.5
	Right lung	Metastasis	Adenocarcinoma	205	416	161	94	268	34.3
	Left lung	Metastasis	Adenocarcinoma	161	252	94	48	314	41.7
	Diaphragm	Metastasis	Adenocarcinoma	102	435	93	44	318	42.9
	Liver	Metastasis	Adenocarcinoma	109	363	124	68	294	50.7
	cfDNA	22		22.8	1170	362			12.8

conc. concentration, VAF variant allele frequency.

^aTumor samples were collected from autopsy.

^bThe sequence depth indicates a median number of that in target lesions.

^cNumber of gene alterations both in tumor and cfDNA are listed. It is the number gene of alterations detected in cfDNA among alterations in tumor.

^dNumber of gene alterations in cfDNA alone are listed. It is the number of alterations detected in cfDNA, not in tumor.