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. 2021 Apr 16;4:481. doi: 10.1038/s42003-021-01999-1

Fig. 3. T8I mutation stabilizes the six-helix bundle and impairs proteolytic processing of cleavage sites flanking SP1.

Fig. 3

a, b Comparison of the CA-SP1 hexamer in GagΔMAT8I (a) and GagΔMAWT (EMD-3782) (b), shown in a side-view. The density map is overlaid with the respective atomic model. The dashed arrows points to the position of the eighth aminoacid in SP1 in both maps. c An enlarged view of density overlay from the dashed box region in (a, b). The GagΔMAT8I density is in grey and the GagΔMAWT density is in blue. The red arrow points to the same central density shown in Fig. 2d. The green arrow points to BVM density in EMD-3782. d Overlay of the refined atomic models from GagΔMAT8I and GagΔMAWT (PDB 5L93). Arrow points to location of T8I mutation and CA|SP1 and SP1|NC cleavage sites. e, f In vitro protease cleavage assays. GagΔMA WT and GagΔMAT8I assemblies were incubated with recombinant HIV-1 PR at the indicated concentration (e) and for the indicated period of time (f), separated in an SDS-PAGE and stained with Coomassie Blue. For processing intermediates, bands are labelled by listing the first and last domains.