Skip to main content
. 2021 Apr 16;12:2286. doi: 10.1038/s41467-021-22642-x

Fig. 3. Mfsd2b is likely a promiscuous S1P transporter.

Fig. 3

a Levels of individual S1P species from resting and thrombin-activated WT and Mfsd2b KO platelets. These are major S1P species found in platelets. Data are mean and SD. Each dot represents one mouse (n = 5). ***P < 0.001. One-way ANOVA was used. b comparison of the levels of each S1P species measured from resting and thrombin-activated WT and KO platelets, respectively. There was a reduction in S1P levels in WT platelets at activated conditions (right panel). Thrombin-activation did not reduce S1P levels in Mfsd2b KO platelets (left panel). Data are mean and SD. Each dot represents on mouse (n = 5). ***P < 0.001; ns not significant. One-way ANOVA was used. WT wild type, KO knockout. c, d Levels of sphingomyelins (SM), ceramides, and sphingosines found in WT and Mfsd2b KO platelets before and after thrombin activation, respectively. Levels are the sum of all measured molecular species in each lipid class. The levels of ceramides and sphingosines were slightly elevated in KO platelets. However, note that the degree of S1P accumulation is much greater compared with ceramides and sphingosines. Data are mean and SD. Each dot represents one mouse (n = 5). ***P < 0.001, two-tailed unpaired t-test was used. Individual lipid species can be found in the supplementary data 1. SM sphingomyelin.