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. 2021 Apr 16;12:2286. doi: 10.1038/s41467-021-22642-x

Fig. 4. Platelets play a dispensable role for plasma S1P.

Fig. 4

a, b Western blot analysis of Mfsd2b expression in platelets and erythrocytes from WT, Mfsd2bf/fPF4, and Mfsd2bf/fEpoR mice. Experiments were performed at least twice with n = 3. WT wild-type, KO knockout, RBC red blood cells. c Extracellular and intracellular S1P levels from transport assays with thrombin-activated platelets from the indicated mice to evaluate the ability of S1P export. Similar to platelets from global knockout of Mfsd2b, platelets from PF4-Cre specific knockout of Mfsd2b (Mfsd2bf/fPF4) had a reduced S1P transport activity comparable to platelets isolated from global KO and control (Mfsd2bf/f). Data are mean and SD. Experiments were performed at least twice with n = 4. ***P < 0.001; ns not significant. One-way ANOVA was used. d Extracellular and intracellular S1P levels from transport assays of isolated erythrocytes to evaluate the ability of S1P export. Erythrocytes isolated from Mfsd2bf/fPF4 had normal S1P transport activity, whereas S1P export activity is reduced in global Mfsd2b KO erythrocytes. Data are mean and SD. Experiments were performed at least twice with n = 4. ***P < 0.001; ns not significant. One-way ANOVA was used. e Total plasma S1P levels from WT (n = 6), global Mfsd2b KO (n = 6), Mfsd2bf/fPF4 (n = 6), Mfsd2bf/fEpoR (n = 6) knockout mice, and Mfsd2bf/f mice (n = 10). Data are mean and SD. ***P < 0.001. ns not significant. One-way ANOVA was used. f S1P levels in platelets isolated from the indicated genotypes. Data are mean and SD. Each dot represents one animal (n = 4). ***P < 0.001; ns not significant. One-way ANOVA was used. DPM disintegrations per minute.