Fig. 6. Mfsd2b knockout platelets had abnormal morphology.

a, b Representative images from scanning electron microscopic (SEM) of resting WT and Mfsd2b KO platelets. Note that KO platelets exhibited membrane damages (shown in yellow arrowheads). WT wild-type, KO knockout. c, d Representative images from transmission electron microscopic (TEM) of resting WT and KO platelets. Note that KO platelets exhibited increased membrane protrusion and blebbing, likely due to defects in the open canalicular systems (shown in red arrows). There were multiple small open canalicular systems (OCS) observed in KO cells (red arrowheads). e, f Representative images from scanning electron microscopic (SEM) of calcium ionophore A23187 (1 µM) activated WT and KO platelets. Platelets were activated for 5 min on coverlips and fixed. Note that activated KO platelets had reduced spreading and increased lamellipodia (shown in yellow arrows). In a–d, representative images from 3 WT and 3 KO mice. In e, f upper and lower panels are images from platelets isolated from two different animals (n = 2).