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. 2021 Apr 16;12:2308. doi: 10.1038/s41467-021-22589-z

Fig. 1. An inducible Nlrc5 (CITA) approach to force DP thymocytes to express MHC Class I molecules.

Fig. 1

a Schematic representation of positive selection of innate-like T cells on DP thymocytes. b mRNA expression level of murine Nlrc5 in different T-cell subsets from B6 WT mice. Data were obtained from ImmGen. c Flow cytometry analysis of HEK 293 cells transduced with lentivirus expression vector encoding the murine Nlrc5 coding sequence (CDS) or with empty virus as a control. As additional controls, non-transduced cells and isotype control staining are shown. Cells are analyzed 48 h post transduction. d Structure of the wild-type (WT) Rosa26 and the targeted allele of the transgenic mouse (Nlrc5-stopflox). e Flow cytometry evaluation of MHC I and MHC II expression on T cells from WT, CD4-Cre × Nlrc5-stopflox (T-MHC I), and Plck-CIITA (T-MHC II) transgenic mice. Data are representative of five independent experiments, n ≥ 5 mice per experimental group. Source data are provided as a Source Data file.