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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Semin Cell Dev Biol. 2020 Oct 17;114:93–112. doi: 10.1016/j.semcdb.2020.09.014

Figure 3. Mammary organoid cultures can replicate characteristics of normal development.

Figure 3.

Representative images of mammary organoid culture derived from pre-pregnancy MECs (Balb/C mice), grown with either essential media or media with pregnancy hormones (containing estrogen (E2), progesterone (P4), and prolactin (Prl). (a) H&E stained organoids (b) light microscope image of organoids in culture (c) Immunofluorescence image of an organoid showing the branching morphogenesis phenotype (d) Immunofluorescence image of an organoid treated with pregnancy hormones expressing Csn2, a milk protein. Scale: 200 µm. Mammary organoid growth conditions and IF staining were performed as described on [198]. Images credits to Chen Chen and Michael F. Ciccone.