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. 2021 Apr 16;12:2294. doi: 10.1038/s41467-021-22276-z

Fig. 3. Design of cyclic crowns from heterodimeric building blocks.

Fig. 3

a Hetero-dimeric HB (green/yellow) fused with different DHRs (shades of blue) were fused together using WORMS by enforcing a specific overall cyclic symmetry (C3 and C5 shown). b The backbones of the crystal structure (yellow/white) of C3_Crn-05 overlaid with the design model (purple/gray). Insets show the backbone matching focused at each of the fusion locations. c A C5 crown (C5_Crn-07, asymmetric unit in red) was fused to DHR units on either outward facing (“C5_Crn_HF-12”, blue arrow) or inward facing termini (“C5_Crn_HF-26”, dark blue arrow). The two structures were then merged together to generate a double fusion (“C5_Crn_HF-12_26”, black arrow). d Cryo-EM class average of the fused 12_26 structure; the major C5 species shown. 3D reconstruction shows the main features of the designed structure are present, as is also evident in the class average (right).