Table 1.

Results of the gene mutation analysis.

	Results of EGFR mutation diagnostic tests								In house NGS (Amplicon Seq)
	Cobas®				Scorpion-ARMS
	JFCR-226	JFCR-065	PC9	A431	JFCR-226	JFCR-065	PC9	A431	JFCR-226
EGFR exon18 G719X	×	×	×	×	×	×	×	×	EGFR: c2239_2240TT > CC (p.L747P)
EGFR exon19 deletions	〇	〇	〇	×	〇	〇	〇	×
EGFR exon20 S768I	×	×	×	×	×	×	×	×
EGFR exon20 Insertions	×	×	×	×	×	×	×	×
EGFR exon20 T790M	×	×	×	×	×	×	×	×
EGFR exon21 L858R	×	×	×	×	×	×	×	×
EGFR exon21 L861Q	×	×	×	×	×	×	×	×

〇; positive, ×; negative.

PC9; EGFR exon19 deletion positive NSCLC cell line.

JFCR-065; EGFR exon19 deletion positive NSCLC cell line.

A431; EGFR wild (EGFR gene amplified).

(a) Comparison of LCC226, LCC065 (patient-derived cells expressing EGFR 19 deletions), PC9 (cells expressing EGFR 19 deletions), and A431 (human squamous carcinoma cells harboring wildtype EGFR gene amplification) by Cobas® and Scorpion-ARMS® EGFR gene mutation analysis results and LCC226 NGS analysis results.