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. 2021 Apr 16;12:2281. doi: 10.1038/s41467-021-22450-3

Fig. 6. Blockade of the MEK/ERK Pathway reduces proliferation and induces apoptosis in Tumor Tissues of AOM/DSS-treated mice.

Fig. 6

a Schema of administration of various inhibitors in AOM/DSS-treated mice. b–f After induction of colorectal tumors, wild-type mice were not treated or treated with Abx for 8 weeks (b), NAC for 4 weeks (c), or trametinib at −6 and −30 h (d–f) (before sacrifice). On day 98–105 after AOM injection, mRNA was extracted from tumor (T) and non-tumor tissues (N), and Il11 expression was determined by qPCR (b–d). Results are mean ± SEM. n = 9 (untreated), 8 (nontumor, Abx-treated), or 7 (nontumor, Abx-treated) mice; pooled data from two independent experiments (b). n = 13 (untreated) or 8 (NAC-treated) mice; pooled data from two independent experiments (c). n = 10 (nontumor, untreated), 11 (tumor, untreated), 9 (nontumor, trametinib-treated), or 10 (nontumor, trametinib-treated) mice; pooled data from four independent experiments (d). Colon tumor sections were stained with anti-Ki67 or anti-CC3 antibodies (e). Ki67+ area and total area were calculated and the percentages of Ki67+ area per area are expressed (f). n = 8 (untreated) or 7 (trametinib-treated) mice. Numbers of CC3+ cells were counted and the total area was calculated, and CC3+ cells per area are expressed as arbitrary units (a.u.) (f). n = 8 (untreated) or 6 (trametinib-treated) mice. Statistical significance was determined using the unpaired two-tailed Student’s t-test (f) and two-way ANOVA with Tukey’s multiple comparison test (b–d). Source data are provided as a Source Data file.