Fig. 7. Blockade of the MEK/ERK Pathway reduces proliferation and induces apoptosis in Tumor Tissues of Apc^Min/+ mice.

a Schema of administration of various inhibitors to Apc^Min/+ mice. b–i Apc^Min/+ mice were not treated or treated with Abx for 8 weeks (b), NAC for 4 weeks (c), or trametinib at –6 and –30 h (d–i), then sacrificed 20–24 weeks after birth. b–d Il11 expression in tumors and nontumor tissues in the colon was determined using qPCR. Results are mean ± SEM. n = 8 (nontumor, untreated), 6 (tumor, untreated), 7 (nontumor, Abx-treated) or 4 (tumors, Abx-treated) mice; pooled data from three independent experiments. n = 4 (untreated) or 6 (NAC-treated) mice; pooled data from two independent experiments. n = 5 (untreated) or 4 (trametinib-treated) mice; pooled data from three independent experiments. Colon (e) or small intestine (g) tumor tissue sections were stained with anti-Ki67 or anti-CC3 antibodies, and analyzed and are expressed as in Fig. 6f, g (f, h). Results are mean ± SEM (n = 5 mice). Il11 expression in tumors and nontumor tissues in the small intestine was determined using qPCR (i). Results are mean ± SEM. n = 4 (untreated) or 7 (trametinib-treated) mice; pooled data from three independent experiments. Statistical significance was determined using the two-way ANOVA with Tukey’s multiple comparison test (b–d, i), or two-tailed unpaired Student’s t-test (f, h). Source data are provided as a Source Data file.