Fig. 1. The complex formation of p53 and BCL-xL.

a Overall structure of p53/BCL-xL complex. An asymmetric unit of p53/BCL-xL complex comprises two p53-DBD molecules (colored cyan and limon) and two BCL-xL molecules (colored magenta and wheat). b A surface model of the ternary complex composed of the p53 dimer and BCL-xL-C. BCL-xL-C molecule inserted deep into the groove formed by the p53 dimer. c A surface model of the ternary complex composed of the p53 dimer and BCL-xL-D. BCL-xL-D molecule sits outside the groove formed by the p53 dimer. d The complex formation of p53-DT and BCL-xL analyzed by protein crosslinking and SDS-PAGE. Purified p53-DT, BCL-xL or their mixture were incubated in the presence or absence of 0.1% glutaraldehyde (GA) as indicated. Products of the crosslinking reactions were analyzed by SDS-PAGE followed by silver staining. The experiments were repeated independently more than 3 times with similar results and a representative experiment is shown. e The binding affinity between p53-DT and BCL-xL was measured using isothermal titration calorimetry (ITC) assays. The binding parameters indicate average of three independent titrations ± SEM. Source data are provided as a Source Data file.