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. 2021 Apr 16;12:2304. doi: 10.1038/s41467-021-22561-x

Fig. 6. Trafficking of mitochondria and DCVs to AIY axons is required for juglone-induced FLP-1 secretion.

Fig. 6

a Representative images and linescans of fluorescence distribution in AIY axons of animals co-expressing FLP-1::Venus (to mark DCVs) and TOMM-20::mCherry (to mark mitochondria) in the indicated mutants. Arrowheads mark fluorescent puncta in AIY axons and arrows mark AIY somas. Scale bar: 10 μm. b Quantification of average coelomocyte fluorescence intensity of the indicated mutants expressing FLP-1::Venus in AIY following 10 min juglone treatment. The boxes span the interquartile range, median is marked by the line and whiskers indicate the minimum and the maximum values. n = 30 biologically independent samples. ***P < 0.001 by Student’s two-tailed t-test. ##P < 0.01, n.s not significant by one-way ANOVA with Dunnett’s test. c Quantification of average coelomocyte fluorescence intensity of the indicated mutants expressing FLP-1::Venus in AIY following 10 min H2O2 treatment or 1 min mito-miniSOG activation. The boxes span the interquartile range, median is marked by the line and whiskers indicate the minimum and the maximum values. n = 30 biologically independent samples. ***P < 0.001 by Student’s two-tailed t-test. ###P < 0.001, n.s not significant by one-way ANOVA with Dunnett’s test.