Fig. 5.
Direct detection of SARS-CoV-2 in crude saliva samples by dWS-CRISPR assay. (a) Workflow for direct SARS-CoV-2 testing in spiked saliva samples by dWS-CRISPR assay. (b) Endpoint fluorescence micrographs of the chip for direct detection of SARS-CoV-2 virus spiked in saliva samples. Saliva samples 1–5, the samples with 10%, 5%, 2.5%, 1%, and 0% of heat-inactivated SARS-CoV-2 virus. Each micrograph is a representative of six distinct regions taken to cover about 2809 microreactions. PC, SARS-CoV-2-positive control sample. NC, SARS-CoV-2-negative control sample. NTC, non-template control. Scale bars are 300 μm.
