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. 2021 Apr 17;17:38. doi: 10.1186/s13223-021-00543-4

Fig. 5.

Fig. 5

Effect of IL-35 on PMA plus A23187-stimulated MAPKs activation. a After pretreatment of IL-35 for 6 h, HMC-1 cells were stimulated by PMA (50 nM) plus A23187 (1 μM) for 15 min for MAPKs activation. Phosphorylation of ERK1/2, JNK1/2, and p38 MAPKs was analyzed by western blotting. b The intensity was measured by densitometry. All data are expressed as mean ± SD. P values are based on the One-way analysis of variance. n = 10. ****P < 0.0001 compared with PMA plus A23187 group