Fig. 1.

Mutated TriBAFF could bind but not activate BAFFR-bearing B cell lines. a PBMCs from two B-ALL patients were analyzed with CD19 and BAFFR using flow cytometry. b K562, Raji, BALL-1, and Jeko-1 were analyzed with CD19 and BAFFR using flow cytometry. c WT and mutated BAFF or TriBAFF were compared. d The binding affinity of WT and mutated BAFF or TriBAFF protein with Raji was detected using flow cytometry. e Proliferations of Raji were compared after adding WT and mutated BAFF or TriBAFF protein (5 µg/mL) into the culture medium