Fig. 2.

BEAST maximum clade credibility tree of Equisetum based on four plastid regions, showing mean ages and 95 % HPD intervals for node ages (grey bars in Myr). Node numbers are indicated below branches in italics. Vertical dashed grey lines separate the four time slices (TSI to TSIV) used in the stratified ancestral range reconstruction DEC analysis (M1). Branch colours represent the estimated ancestral genome sizes (GS, in pg/1C value; Supplementary Data Table S3) reconstructed using Phytools based on the mean 1C values obtained from extant species, which range from 11.90 to 31.34 pg/1C (Table 3) as shown in the legend. We also provide the estimated genome sizes for the MRCA of all extant Equisetum species and those for the MRCA of subgenera Equisetum and Hippochaete (pg/1C value; Supplementary Data Table S3).