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. Author manuscript; available in PMC: 2021 Apr 17.
Published in final edited form as: J Am Chem Soc. 2020 May 22;142(22):9999–10007. doi: 10.1021/jacs.0c01325

Figure 1.

Figure 1.

5-AR glycates histones in vitro. (A) Schematic depicting incubation of histones with probes followed by click labeling with DBCO Cy5. (B) Incubation of recombinant histones with PBS, 5 mM of d-ribose, 1-AR, or 5-AR for 8 h followed by click-labeling and in-blot fluorescence analysis. (C) Histones glycated with 5 mM of 5-AR, labeled with DBCO Cy5, analyzed side-by-side by in-blot fluorescence and Coomassie Brilliant Blue (CBB) for loading control.