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. Author manuscript; available in PMC: 2021 Apr 17.
Published in final edited form as: Neuron. 2019 Feb 5;101(4):721–737.e4. doi: 10.1016/j.neuron.2019.01.015

Figure 8. Tetraspanins are functionally critical SREBP effectors.

Figure 8.

(A) A small group of genes display significantly (q sig) altered expression between flies expressing Rh1-Gal4 >> UAS-mCD8-GFP versus Rh1-Gal4 >> UAS-SREBP1-452. (B) Control log10 FPKM values versus log2-fold change in expression. (C) Log2-fold change in FPKM values from RNA-seq in the SREBP1-452 condition relative to control for lipid biosynthetic genes, synaptic genes, and eye and phototransduction-related genes. (D-G) Lamina cross sections stained with an antibody directed against CSP. (D) Control flies in which UAS-mCD8-GFP is driven by Rh1-Gal4. (E) Flies expressing UAS-SREBP1-452 driven by Rh1-Gal4. (F) Flies co-expressing UAS-SREBP1-452 with EP:Tsp39D driven by Rh1-Gal4. (G) Flies expressing UAS-SREBP1-452 and EP:Tsp42Eo driven by Rh1-Gal4. (H) Flies expressing eye-specific eas-IR. (I) Flies co-expressing eye-specific eas-IR and EP Tsp39D. (J) Quantification of relative CSP labeling. Data represented as mean ± SEM. Differences significant at p < 0.001 (***) (one-way ANOVA). Scale bar: 15 µm. n = 9 for each genotype. See also Figure S8 and Supplemental Table 2.