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. 2021 Mar 27;49(7):4085–4103. doi: 10.1093/nar/gkab190

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Clustering of 20-nt rRFs from 18S rRNAs reveals potential binding modes. (A) Base-pairing patterns of the 20-nt rRFs in 4603 unique rRF-target interactions. (B) Sequence logos show the nucleotide composition patterns for unique rRF isoforms in every cluster. (C) Base-pairing patterns of four 18S rRFs with the most interactions with different target genes. Sequence logos below show the frequency of hybridization with the target at each rRF position. The arrows show the frequency (darker shade being more frequent) of T→C conversions in PAR-CLIP data (see section E).